Studying Microparticles on the ISSLabSmith application note
A team from the University of Louisville will be using LabSmith uProcess microfluidic components and Microscopes to study microparticle suspension on the International Space Station (ISS). … Read more
Field Amplified Sample Stacking (FASS) for Sample Concentration in a NanochannelElizaveta Davies, Lesa Bishop, Adam Lucio, Jackson Travis del Bonis-O'Donnell, Sumita Pennathur, University of California, Santa Barbara; Yolanda Fintschenko, LabSmith, Inc.
A ubiquitous problem in analytical chemistry is the push to increase signal-to-noise (S/N) and to lower the limits of detection (LOD) for many sample separation methods. Low S/N is typically due to the inherent low concentration of the sample in the system and the band-broadening of the sample in dead volumes of the analytical system. … Read more
Performing an Electrokinetic Gated Injection in a NanochannelElizaveta Davies, Lesa Bishop, Adam Lucio, Travis del Bonis O'Donnell, Sumita Pennathur, University of California, Santa Barbara; Yolanda Fintschenko, LabSmith, Inc.
The microfluidic chip platform offers an advantage for electrokinetic chemical separations by eliminating the dead volume normally encountered with tubing connections. In order to capitalize on this advantage, sample injections must be performed on chip. The gated injection loads a variable injection volume by controlling velocity and injection time. The gated injection controls plug size … Read more
Making a Pinched Microfluidic Injection with the LabSmith LabPackage-Making a Microfluidic Injection on a ChipYolanda Fintschenko and Kirsten Pace LabSmith, Inc.
LabSmith’s LabPackage makes it easy to build, control and monitor simple and complex microfluidic manipulations. This experiment was designed to help you train your laboratory team and test competency on performing a pinched electrokinetic microfluidic injection techniques. It can also be used to test equipment, chips and reagents to ensure that they are working properly. … Read more
LabSmith Microparticle Image Velocimetry for Improving Dielectrophoretic Concentrator Using the LabSmith LabPackageJosé I. Martinez-López, Hector Moncada-Hernández, Javier L. Baylon-Cardiel, Sergio O. Martinez-Chapa, Tecnológico de Monterrey (Monterrey NL, Mexico) Blanca H. Lapizco-Encinas, CINVESTAV-Monterrey (Apodaca NL, Mexico), and Yolanda Fintschenko, LabSmith.
Sample preparation is a bottleneck for protein, cell and nucleic acid analysis, regardless of the analytical technique used. An important, and tedious, preparation step is selective concentration of the target of interest, particularly when target abundance is low. One approach is insulator-based dielectrophoresis, or iDEP, in which insulators, often etched in glass or stamped in … Read more
Prediction of trapping zones in an insulator-based dielectrophoretic deviceJavier L. Baylon-Cardiel , Blanca H. Lapizco-Encinas , Claudia Reyes-Betanzo , Ana V. Chávez-Santoscoy and Sergio O. Martínez-Chapa
Baylon-Cardiel, J.L.; Lapizco-Encinas,B.L.;Reyes-Betanzo,C.; Chavez-Santoscoy,A.V.; Martınez-Chapa, S.O. Prediction of trapping zones in an insulator-based dielectrophoretic device Lab-on-a-Chip, 2009, 9, 2896–2901. Abstract A mathematical model is implemented to study the performance of an insulator-based dielectrophoretic device. The geometry of the device was captured in a computational model that solves Laplace equation within an array of cylindrical insulating … Read more
Characterization of electrokinetic mobility of microparticles in order to improve dielectrophoretic concentrationJosé I. Martínez-López, Héctor Moncada-Hernández, Javier L. Baylon-Cardiel, Sergio O. Martínez-Chapa, Marco Rito-Palomares and Blanca H. Lapizco-Encinas
Martínez-López , J.I. ; Moncada-Hernández, H.;. Baylon-Cardiel, J. L.; Martínez- Chapa, S. O.; Rito-Palomares, M. ; Lapizco-Encinas, B.H., Characterization of electrokinetic mobility of microparticles in order to improve dielectrophoretic concentration, Anal Bioanal. Chem. 2009,394, 293–302. Abstract Insulator-based dielectrophoresis (iDEP), an efficient technique with great potential for miniaturization, has been successfully applied for the manipulation of … Read more
Protein manipulation with insulator-based dielectrophoresis and direct current electric fieldsBlanca H. Lapizco-Encinas, Sandra Ozuna-Chacón, Marco Rito-Palomares
Lapizco-Encinas, B.H.; Ozuna-Chaco´, S.; Rito-Palomares, M.; Protein manipulation with insulator-based dielectrophoresis and direct current electric fields, J.Chrom. A, 2008, 1206, 45–51. Abstract The present study demonstrates the manipulation of protein particles employing insulator-baseddielectrophoresis (iDEP) and directcurrent (d.c.) electricfields. Fluorescently labeled bovine serum albumin (BSA) protein particles were concentrated inside a microchannel that contained an array … Read more