Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature
Alireza Salmanzadeh, Lina Romero, Hadi Shafiee, Roberto C. Gallo-Villanueva, Mark A. Stremler, Scott D. Cramer and Rafael V. Davalos November 9, 2011
Salmanzadeh, A.; Romero, L. Shafiee, H.; Gallo-Villanueva, R.C.; Stremler, M.A.; Cramer, S.D.; Davalos, R.D.; Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature. Lab Chip, 2012,12, 182-189.
In this study, the dielectrophoretic response of prostate tumor initiating cells (TICs) was investigated in a microfluidic system utilizing contactless dielectrophoresis (cDEP). The dielectrophoretic response of prostate TICs was observed to be distinctively different than that for non-TICs, enabling them to be sorted using cDEP. Culturing the sorted TICs generated spheroids, indicating that they were indeed initiating cells. This study presents the first marker-free TIC separation from non-TICs utilizing their electrical fingerprints through dielectrophoresis.