Isolation of Prostate Tumor Initiating Cells

Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature

Alireza Salmanzadeh et al November 9, 2011

Salmanzadeh, A.; Romero, L. Shafiee, H.; Gallo-Villanueva, R.C.; Stremler, M.A.; Cramer, S.D.; Davalos, R.D.; Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature. Lab Chip, 2012,12, 182-189.

Abstract

In this study, the dielectrophoretic response of prostate tumor initiating cells (TICs) was investigated in a microfluidic system utilizing contactless dielectrophoresis (cDEP). The dielectrophoretic response of prostate TICs was observed to be distinctively different than that for non-TICs, enabling them to be sorted using cDEP. Culturing the sorted TICs generated spheroids, indicating that they were indeed initiating cells. This study presents the first marker-free TIC separation from non-TICs utilizing their electrical fingerprints through dielectrophoresis.

 
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Application: Dielectrophoretics