Applying Voltage to a Channel in a Microfluidic Chip

Platinum wire electrodes are commonly used to apply voltage to a fluidic channel. Although the approach is relatively simple, proper preparation of the electrode is very important to prevent field concentrations in the channels. Field concentrations can cause a multitude of problems, including bubble formation, cell lysing, localized heating, chemical reactions, and clogging of channels.

Lab-on-a-Chip uProcess™ Application: Continuous Dispensing

Particularly in biological experiments, it is necessary to deliver fluid at flow rates of ≤ 1μl/min over experiment times of hours to days.  As a result, a relatively large total volume is dispensed to the experiment, sometimes as much as 1-10 ml. In lab-on-a-chip experiments, a small overall experimental platform is often desired. This creates the challenge of … Read more

Lab-on-a-Chip Application: Circulating Tumor Cell Immuncapture Automation

The manipulation of biological samples for diagnostic purposes requires reproducibility and simplicity to be useful in practice. As microfluidic devices transition towards use in medical diagnostics, traditional handheld syringe pumps can be too bulky and laborious for medical procedures that require quick turnaround times. Here, we discuss the evaluation of a portable breadboard platform that … Read more

Making a Pinched Microfluidic Injection with the LabSmith LabPackage-Making a Microfluidic Injection on a Chip

LabSmith’s LabPackage makes it easy to build, control and monitor simple and complex microfluidic manipulations. This experiment was designed to help you train your laboratory team and test competency on performing a pinched electrokinetic microfluidic injection techniques. It can also be used to test equipment, chips and reagents to ensure that they are working properly. … Read more

Microfluidic Polyacrylamide Gel Electrophoresis with In-situ Immunoblotting for Native Protein Analysis

He, M.; Herr, A.E. “Microfluidic Polyacrylamide Gel Electrophoresis with In-situ Immunoblotting for Native Protein Analysis.” Analytical Chemistry, 2009, 81, 8177-84. Abstract We introduce an automated immunoblotting method that reports protein electrophoretic mobility and identity in a single streamlined microfluidic assay. Native polyacrylamide gel electrophoresis (PAGE) was integrated with subsequent in situ immunoblotting. Integration of three … Read more