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Gradient Elution Microchannel Electrochromatography

Separations of complex peptide mixtures can take too long to elute without the ability to apply a solvent gradient. One solution for applying a gradient is called Microchannel Electrochromatography, or MEC. In reversed phase MEC buffers with aqueous and organic components comprise the mobile phase of a separation through a solid and porous stationary phase … Read more

Gradient Elution in Micro Channel Electrochromatography

Watson, M.W.L.; Mudrik, J.M.;Wheeler,A.R., Gradient elution in micro channel electrochromatography, Anal.Chem., 2009, 81, 3851-3857. Abstract There is great interest in using microfluidic channels packed with a stationary phase for chemical separations of complex mixtures. A key advantage of such techniques is the use of electroosmotic flow (EOF), controlled simply by applying electrical potentials between reservoirs. … Read more

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