Lab-on-a-Chip Application: Microfab-less Microfluidics for a Portable Hybrid Microchip-Capillary Electrophoresis Device

Microfluidic devices, with their potential for portability and rapid analysis times, can bring cost-effective flexibility to modern analytical chemistry applications. However, the design and fabrication of microchips can involve trade-offs between cost, flexibility of configuration, and ideal surface properties or performance. To circumvent these drawbacks, we have developed a hybrid platform consisting of simple microfabricated … Read more

Applying Voltage to a Channel in a Microfluidic Chip

Platinum wire electrodes are commonly used to apply voltage to a fluidic channel. Although the approach is relatively simple, proper preparation of the electrode is very important to prevent field concentrations in the channels. Field concentrations can cause a multitude of problems, including bubble formation, cell lysing, localized heating, chemical reactions, and clogging of channels.

Performing an Electrokinetic Gated Injection in a Nanochannel

The microfluidic chip platform offers an advantage for electrokinetic chemical separations by eliminating the dead volume normally encountered with tubing connections. In order to capitalize on this advantage, sample injections must be performed on chip. The gated injection loads a variable injection volume by controlling velocity and injection time. The gated injection controls plug size … Read more

Making a Pinched Microfluidic Injection with the LabSmith LabPackage-Making a Microfluidic Injection on a Chip

LabSmith’s LabPackage makes it easy to build, control and monitor simple and complex microfluidic manipulations. This experiment was designed to help you train your laboratory team and test competency on performing a pinched electrokinetic microfluidic injection techniques. It can also be used to test equipment, chips and reagents to ensure that they are working properly. … Read more

LabSmith Microparticle Image Velocimetry for Improving Dielectrophoretic Concentrator Using the LabSmith LabPackage

Sample preparation is a bottleneck for protein, cell and nucleic acid analysis, regardless of the analytical technique used. An important, and tedious, preparation step is selective concentration of the target of interest, particularly when target abundance is low. One approach is insulator-based dielectrophoresis, or iDEP, in which insulators, often etched in glass or stamped in … Read more

Microfluidic Polyacrylamide Gel Electrophoresis with In-situ Immunoblotting for Native Protein Analysis

He, M.; Herr, A.E. “Microfluidic Polyacrylamide Gel Electrophoresis with In-situ Immunoblotting for Native Protein Analysis.” Analytical Chemistry, 2009, 81, 8177-84. Abstract We introduce an automated immunoblotting method that reports protein electrophoretic mobility and identity in a single streamlined microfluidic assay. Native polyacrylamide gel electrophoresis (PAGE) was integrated with subsequent in situ immunoblotting. Integration of three … Read more

Prediction of trapping zones in an insulator-based dielectrophoretic device

Baylon-Cardiel, J.L.; Lapizco-Encinas,B.L.;Reyes-Betanzo,C.; Chavez-Santoscoy,A.V.; Martınez-Chapa, S.O. Prediction of trapping zones in an insulator-based dielectrophoretic device Lab-on-a-Chip, 2009, 9, 2896–2901. Abstract A mathematical model is implemented to study the performance of an insulator-based dielectrophoretic device. The geometry of the device was captured in a computational model that solves Laplace equation within an array of cylindrical insulating … Read more