LabSmith Applications

LabSmith's products are made by researchers, for researchers. We know the value of having just the right tools in the lab, letting you focus on science.
Protein manipulation with insulator-based dielectrophoresis and direct current electric fields

Protein manipulation with insulator-based dielectrophoresis and direct current electric fields

By Blanca H. Lapizco-Encinas, Sandra Ozuna-Chacón, Marco Rito-Palomares on October 3, 2008.


Lapizco-Encinas, B.H.; Ozuna-Chaco´, S.; Rito-Palomares, M.; Protein manipulation with insulator-based dielectrophoresis and direct current electric fields, J.Chrom. A, 2008, 1206, 45–51.

Abstract

The present study demonstrates the manipulation of protein particles employing insulator-baseddielectrophoresis (iDEP) and directcurrent (d.c.) electricfields. Fluorescently labeled bovine serum albumin (BSA) protein particles were concentrated inside a microchannel that contained an array of glass cylindrical insulating structures. d.c. electricfields were applied and the dielectrophoretic response of the particles was observed as a function of the suspending medium conductivity (25, 50 and 100 μS/cm) and pH (8 and 9). It was shown that the magnitude of the applied electricfield (700–1600 V/cm) and suspending medium properties have a strong effect on the dielectrophoretic response of the protein particles. The results presented here are the first report on proteinmanipulation employing d.c.-iDEP.