Dependence of Nonlinear Electrophoresis on Particle Size and Electrical Charge

ABSTRACT This study focuses on the dependence of nonlinear electrophoretic migration of particles on the particle size and particle electrical charge. This is the first report of the experimental assessment of the mobilities of the nonlinear electrophoretic velocity of colloidal polystyrene microparticles under two distinct electric field dependences. A total of nine distinct types of … Read more

Analytical Electrolyzer for Characterization of Flow Plates

ABSTRACT We present an analytical electrolyzer with sensors embedded within flow plates to enable direct measurement of electrolyte temperatures and pressures in real time during water electrolysis. Flow plates with either parallel or serpentine channels and a total of eight equally spaced sensors were integrated into a flow cell containing a nickel foam gas diffusion … Read more

Microfluidic Molecular Separation for Purine Derivatives Analysis

Abstract Based on its highly specific and non-invasive features, surface-enhanced Raman scattering (SERS) has been applied for analytical chemistry or biological applications, such as identification of chemical compositions, cells or bacteria. However, if the targeted sample consists of multiple compounds, the corresponded SERS spectra would be quite difficult to analyze. To address above problems, we … Read more

Portable MPLC using LabSmith’s uProcess Components

A research group led by Dr. Mirek Macka at University of Tasmania has published a journal article on their work using LabSmith’s uProcess components to develop portable medium pressure capillary liquid chromatography instrumentation.   Yan Li, Miloš Dvořák, Pavel N. Nesterenko, Roger Stanley, Nantana Nuchtavorn, Lenka Kujovská Krčmová, Jana Aufartová, Mirek Macka. Miniaturised medium pressure capillary liquid … Read more

Isolation of Prostate Tumor Initiating Cells

Salmanzadeh, A.; Romero, L. Shafiee, H.; Gallo-Villanueva, R.C.; Stremler, M.A.; Cramer, S.D.; Davalos, R.D.; Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature. Lab Chip, 2012,12, 182-189. Abstract In this study, the dielectrophoretic response of prostate tumor initiating cells (TICs) was investigated in a microfluidic system utilizing contactless dielectrophoresis (cDEP). The dielectrophoretic response … Read more

Automated Immunoblotting for Native Protein Analysis

He, M.; Herr, A.E. “Microfluidic Polyacrylamide Gel Electrophoresis with In-situ Immunoblotting for Native Protein Analysis.” Analytical Chemistry, 2009, 81, 8177-84. Abstract We introduce an automated immunoblotting method that reports protein electrophoretic mobility and identity in a single streamlined microfluidic assay. Native polyacrylamide gel electrophoresis (PAGE) was integrated with subsequent in situ immunoblotting. Integration of three … Read more

Prediction of Trapping Zones

Baylon-Cardiel, J.L.; Lapizco-Encinas,B.L.;Reyes-Betanzo,C.; Chavez-Santoscoy,A.V.; Martınez-Chapa, S.O. Prediction of trapping zones in an insulator-based dielectrophoretic device Lab-on-a-Chip, 2009, 9, 2896–2901. Abstract A mathematical model is implemented to study the performance of an insulator-based dielectrophoretic device. The geometry of the device was captured in a computational model that solves Laplace equation within an array of cylindrical insulating … Read more

Gradient Elution in Micro Channel Electrochromatography

Watson, M.W.L.; Mudrik, J.M.;Wheeler,A.R., Gradient elution in micro channel electrochromatography, Anal.Chem., 2009, 81, 3851-3857. Abstract There is great interest in using microfluidic channels packed with a stationary phase for chemical separations of complex mixtures. A key advantage of such techniques is the use of electroosmotic flow (EOF), controlled simply by applying electrical potentials between reservoirs. … Read more

Improved Dielectrophoretic Concentration for iDEP

Martínez-López , J.I. ; Moncada-Hernández, H.;. Baylon-Cardiel, J. L.; Martínez- Chapa, S. O.; Rito-Palomares, M. ; Lapizco-Encinas, B.H., Characterization of electrokinetic mobility of microparticles in order to improve dielectrophoretic concentration, Anal Bioanal. Chem. 2009,394, 293–302. Abstract Insulator-based dielectrophoresis (iDEP), an efficient technique with great potential for miniaturization, has been successfully applied for the manipulation of … Read more

Protein Manipulation with iDEP

Lapizco-Encinas, B.H.; Ozuna-Chaco´, S.; Rito-Palomares, M.; Protein manipulation with insulator-based dielectrophoresis and direct current electric fields, J.Chrom. A, 2008, 1206, 45–51. Abstract The present study demonstrates the manipulation of protein particles employing insulator-baseddielectrophoresis (iDEP) and directcurrent (d.c.) electricfields. Fluorescently labeled bovine serum albumin (BSA) protein particles were concentrated inside a microchannel that contained an array … Read more

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