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Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature

Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature

By Alireza Salmanzadeh, Lina Romero, Hadi Shafiee, Roberto C. Gallo-Villanueva, Mark A. Stremler, Scott D. Cramer and Rafael V. Davalos on November 9, 2011.


Salmanzadeh, A; Romero, L; Shafiee, H; Gallo-Villanueva, R.C ; Stremler, M.A.; Cramer, S.D.; Davalos, R.D.; Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature. Lab Chip, 2012,12, 182-189.

Abstract

In this study, the dielectrophoretic response of prostate tumor initiating cells (TICs) was investigated in a microfluidic system utilizing contactless dielectrophoresis (cDEP). The dielectrophoretic response of prostate TICs was observed to be distinctively different than that for non-TICs, enabling them to be sorted using cDEP. Culturing the sorted TICs generated spheroids, indicating that they were indeed initiating cells. This study presents the first marker-free TIC separation from non-TICs utilizing their electrical fingerprints through dielectrophoresis.